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ANTIINFLAMMATORY EFFECT OF BETA-BETA (Lunasia amara) in WISTAR RATS KESIMPULAN Bayam duri (Ammaranthus sp)

Authors:

Abstract

Introduction. The Beta-beta (Lunasia amara) extract is being used orally and topically in traditional Indonesia for the treatment of leg swelling, skin diseases and inflammation or irritation of the eyes. The anti-inflammatory effect of beta-beta wood were tested in rats induced Complete Freund’s Adjuvant (CFA) 0,1%. Aim. Test the effect anti-inflammatory of beta-beta wood (Lunasia amara) in Wistar rats. Methods. Anti-inflammatory effects of beta-beta was observed at the foot of Wistar rats induced Complete Freund's Adjuvant (CFA). Animals were divided into 6 groups with each group consisting of 6 animals: the group given extract beta-beta at a dose of 5 mg / kg body weight (BW) and 10 mg/kg BW, group given a fraction of beta-beta and a control group that controls positive, normal controls and negative controls. Antiinflammatory effect was observed through a decrease in volume of edema in rats induced foot CFA. Edema volume measurements using a pletismometer. Results. Beta-beta wood has anti-inflamamtory effect in Wistar rats induced CFA. The measurement results between the group given the extract and the fraction of the beta-beta wood compared with diclofenac sodium control group showed no significant difference (p˂0,05). Conclusion. Beta-beta wood (Lunasia amara) has anti-inflammatory effects in Wistar rats. Keyword : Anti-inflammatory, beta-beta (Lunasia amara), Wistar rats, Complete Freund’s adjuvant (CFA), pletismometer
ANTIINFLAMMATORY EFFECT OF BETA-BETA (
Lunasia amara
) in
WISTAR RATS
Hasnaeni1,4*, Sudarsono1, Nurrochmad A2,, Widyarini S3, Selpida4
1 Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta, Indonesia-55281
2 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta,
Indonesia-55281
3 Department of Pathology Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Karangmalang Yogyakarta,
Indonesia-55281
4 Faculty of Pharmacy, Universitas Muslim Indonesia, Urip Sumoharjo Makassar, 90231
E-mail of corresponding authors : hasnaeniyahya@yahoo.co.id
KESIMPULAN
Bayam duri (Ammaranthus sp)
RESULT
0
50
Sodium
diclofenac
Extract
dose 5
mg/kg
Extract
dose 10
mg/kg
DCM
fraction
dose 5
mg/kg
% Inflammatory
% Inflammatory
REFERENCES :
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5.Arnida, Imono AD, Subagus W., (2003), Isolasi fraksi aktif afrodisiaka dari kayu Sanrego (Lunasia amara Blanco),
MFI, 14(4): 195-200.
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from Ruta graveolens L. Inflamm. Res., 62(4), 367376.
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Anti-inflammatory activity and toxicity studies of Chloroxylon sweitenia in Rats. Anc. Sci. Life, 25(3-4), 3343
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ABSTRACT
Introduction. The Beta-beta (Lunasia amara) extract is being used orally and topically in traditional
Indonesia for the treatment of leg swelling, skin diseases and inflammation or irritation of the eyes. The
anti-inflammatory effect of beta-beta wood were tested in rats induced Complete Freund’s Adjuvant
(CFA) 0,1%.
Aim. Test the effect anti-inflammatory of beta-beta wood (Lunasia amara) in Wistar rats.
Methods. Anti-inflammatory effects of beta-beta was observed at the foot of Wistar rats induced
Complete Freund's Adjuvant (CFA). Animals were divided into 6 groups with each group consisting of 6
animals: the group given extract beta-beta at a dose of 5 mg/kg body weight (BW) and 10 mg/kg BW,
group given a fraction of beta-beta and a control group that controls positive, normal controls and
negative controls. Antiinflammatory effect was observed through a decrease in volume of edema in rats
induced foot CFA. Edema volume measurements using a pletismometer.
Results. Beta-beta wood has anti-inflamamtory effect in Wistar rats induced CFA. The measurement
results between the group given the extract and the fraction of the beta-beta wood compared with
diclofenac sodium control group showed no significant difference (p˂0,05).
Conclusion. Beta-beta wood (Lunasia amara) has anti-inflammatory effects in Wistar rats.
Keyword : Anti-inflammatory, beta-beta (Lunasia amara), Wistar rats, Complete Freund’s adjuvant
(CFA), pletismometer
ACKNOWLEDGEMENT
1. Directorate General of Higher
Education, Ministry of Research
Technology and Higher Education for
provision of BPPDN scholarship.
2. Resources Research and
Development Institute (LP2S)
Universitas Muslim Indonesia
Makassar.
3. Department of Pharmaceutical
Biology and Department of
Pharmacology Faculty of Pharmacy,
Department of Pathology Anatomy,
Faculty of Veterinary Medicine,
Universitas Gadjah Mada on facilities
to conduct research.
4. We are also grateful to Mrs. Yahya
and Baharuddin who assisted with the
collection of samples of beta-beta
wood (Lunasia amara).
Figure 1. Curve percentage inflammation of ethanol extract and
fractions dichloromethane beta-beta wood / sanrego
(Lunasia amara) induced CFA 0.1% for 21 days (n = 6)
Figure 2. Charts the percentage antiinflammatory of extracts and
fractions beta-beta wood/ sanrego (Lunasia amara) in Wistar
rats induced by CFA 0.1% for 21 days (n = 6) p˃0,05
1 Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta, Indonesia-
55281
2 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta,
Indonesia-55281
3 Department of Pathology Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Karangmalang Yogyakarta,
Indonesia-55281
4 Faculty of Pharmacy, Universitas Muslim Indonesia, Urip Sumoharjo Makassar, 90231
E-mail of corresponding authors : hasnaeniyahya@yahoo.co.id
INTRODUCTION
Inflammation is a condition characterized by swelling,
heat, redness, pain and dysfunction. In general, the
inflammatory reaction is common in patients with rheumatoid
arthritis and osteoarthritis. There has been no systematic
research on the prevalence, spectrum and patterns of
chronic joint disease, but are common causes the onset of
disability in adults in developing countries (1). One attempt
to cure this disease is to reduce inflammation by using anti-
inflammatory (2; 3).
Beta-beta wood is one of those plants that have potential
as an anti-inflammatory drug. In South Sulawesi beta-beta
wood or sanrego especially in Bone regency, Sanrego or
beta-beta wood (Lunasia amara) known as a tonic in men
(aprodisia) (4). Study of pharmacological activity of beta-
beta wood plants are lacking, but the pharmacological
activity in particular as anti-inflammatory variety of plant
species has been widely studied family Rutaceae.
METHODS : Anti-inflammatory effects of beta-beta was observed at the foot of Wistar rats induced Complete Freund's Adjuvant (CFA). Animals were divided into
6 groups with each group consisting of 6 animals: the group given extract beta-beta at a dose of 5 mg/kg body weight (BW) and 10 mg/kg BW, group given a fraction of
beta-beta and a control group that controls positive, normal controls and negative controls. Antiinflammatory effect was observed through a decrease in volume of edema
in rats induced foot CFA. Edema volume measurements using a pletismometer.
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