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EFFECT INFLAMMATORY OF LUNASIA AMARA EXTRACT ETHANOLIC ON EXPERIMENTAL CHRONIC ARTHRITIS

Authors:

Abstract

The effect inflammatory of Lunasia amara ethanol extract (LAE) on Freund’s complete adjuvant (FCA)-induced arthritis were investigated. Effect inflammatory assessment was done by measuring – paw volume (hind paw swelling), joint diameter and body weight. The oral administration of LAE (50, 100 and 200 mg/kg BW per day) for 60 days after subcutaneous immunization with FCA, significantly reduced hind paw sweeling and reduced joint diameter compared with the FCA-induced arthritis group. LAE (50, 100 and 250 mg/kg) was orally administered once daily for 60 days after subcutaneous administration with FCA. The arthritis score and paw edema were markedly suppressed in the groups treated with LAE. The result suggest that the Lunasia amara Extract ethanolic might be beneficial in the treatment of chronic inflammatory disorder. Keyword : Lunasia amara extract ethanolic, chronic inflammation, FCA-induced arthritis
EFFECT INFLAMMATORY OF LUNASIA AMARA
EXTRACT ETHANOLIC ON EXPERIMENTAL
CHRONIC ARTHRITIS
Hasnaeni1,2*, Sudarsono2, Arief Nurrochmad3, Sitarina Widyarini4
1Faculty of Pharmacy, Uiversitas Muslim Indonesia, Urip Sumoharjo Makassar, Indonesia 90231
2Department of Pharmaceutical Biology, Faculty of Pharmacy, UGM, Sekip Utara Yogyakarta, Indonesia-55281
3Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, UGM, Sekip Utara Yogyakarta, Indonesia-55281
4Department of Pathology Anatomy, Faculty of Veterinary Medicine, UGM, Karangmalang Yogyakarta, Indonesia-55281
*E-mail : hasnaeniyahya@yahoo.co.id
KESIMPULAN
Bayam duri (Ammaranthus sp) memiliki batang yang basah dan lemah, daunnya
berbentuk segitiga dan bunganya berwarna kuning, buah buni berwarna merah pada waktu tua,
berbiji bany
INTRODUCTION
Beta-beta wood is one of the plants of the genus Lunasia
family Rutaceae. In general, Family Rutaceae containing
compounds essential oils, alkaloids, amides, coumarin,
flavonoids, benzoate acids, tannin substances, lignin,
phenolic compounds, triterpene tetrasiklik, diterpene,
tetraterpen pentacyclic saponins, carbohydrates and slime [1].
Scopoletin compound is one of the compounds contained in
family Rutaceae family is known to have anti-inflammatory,
a compound coumarin derivative which is a derivative of
phenyl propane.
Has done research on beta-beta timber that research on
antiinflammatory activity in mice Balb/c model of
rheumatoid arthritis induced Complete Freund's Adjuvant
(CFA) of 0.1%. In this research, it is known that there is a
decrease in inflammation of 77.35% for all treatments.
MATERIAL AND METHOD
Plant Material
The plant of beta-beta wood (Lunasia amara Blanco.) collected at
Siawung, South Sulawesi province, Indonesia in March 2014.
Extraction
Process extraction of the plant is done by maceration. Beta-beta wood
of 1000 g was extracted by maceration at room temperature with ethanol
96% for 72 hour.
Mode of feeding
The suspension extract a dose of 50 mg / kg BW, 100mg/kg body
weight and 250 mg/kg BW and Sodium diclofenac dose of 2 mg/kg BW
administered once daily for 60 days as per oral with a maximum volume
of 1 ml/20 g.
Arthritis index measurement edema volume used
Pletismometer on day 0, day 7, day 14, day 21 to day 56 after the
induction of Control Freund’s Adjuvant (CFA).
In preparation for observed histopathology and index arthritis,
inflammation and joint damage.
RESULT
Figure 1 Average edema volume vs treatment (Control CFA
the group induced CFA 0,1% without treatment,
normal controls without treatment, Control sodium
diclofenac 2 mg/kg, ethanolic extract dose of 50,
100 and 250 mg / kg
Figure 2. Histologycal : A. normal controls (HE); B. edema
that occurs (HE); C. Perikondritis (HE); D. necrosis due to
chronic inflammation
0
0.2
0.4
0.6
014 28 42 56
Average udema
volume (ml)
Treatment (day)
Control CFA
Control
Normal
CONCLUSION
The result suggest that the Lunasia amara Extract
ethanolic might be beneficial in the treatment of
chronic inflammatory disorder (RA).
Refrence :
[1] Hegnauer, R., 1969, Chemotaxonomi der Planzen, edisi V. Birkkhauser
Verlag. Basel and Stuttgart.
[2] Ding, Z., Dai, Y., Hao, H., Pan, R., Yao, X., dan Wang, Z., 2008. Anti-
Inflammatory Effects of Scopoletin and Underlying Mechanisms.
Pharm. Biol., 46(12), 854860.
[3] Sommerfelt, R. M., Feuerherm, A. J., Jones, K., dan Johansen, B., 2013.
Cytosolic Phospholipase A2 Regulates TNF-Induced Production of Joint
Destructive Effectors in Synoviocytes. PLoS ONE, 8(12).
[4] Kim, H.-J., Jang, S. I., Kim, Y.-J., Chung, H.-T., Yun, Y.-G., Kang, T.-H.,
dkk., 2004. Scopoletin suppresses pro- inflammatory cytokines and PGE2
from LPS-stimulated cell line, RAW 264.7 cells. Fitoterapia, 75(3-4),
261266.
[5] Iaroshenko, V. O., Erben, F., Mkrtchyan, S., Hakobyan, A., Vilches-
Herrera, M., Dudkin, S., dkk., 2011. 4-Chloro-3-(trifluoroacetyl)- and 4-
chloro-3-(methoxalyl)coumarins as novel and efficient building blocks for
the regioselective synthesis of 3,4-fused coumarins. Tetrahedron, 67(41),
79467955.
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The effect inflammatory of Lunasia amara ethanol
extract (LAE) on Freund’s complete adjuvant
(FCA)-induced arthritis were investigated. Effect
inflammatory assessment was done by measuring
paw volume (hind paw swelling), joint diameter and
body weight. The oral administration of LAE (50,
100 and 200 mg/kg BW per day) for 60 days after
subcutaneous immunization with FCA. The arthritis
score and paw edema were markedly suppressed in
the groups treated with LAE. The result suggest that
the Lunasia amara Extract ethanolic might be
beneficial in the treatment of chronic inflammatory
disorder.
Keyword : Lunasia amara extract ethanolic, chronic
inflammation, FCA-induced arthritis
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ACKNOWLEDGEMENT
The first author is grateful to Directorate General of Higher
Education, The Ministry of Education and Culture Indonesia for
Scholarship BPPDN Asistance.
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