ELISA Test on Cordia myxa L. Leaf Extract for α-Glucosidase Inhibitor

Handayani, Virsa (2019) ELISA Test on Cordia myxa L. Leaf Extract for α-Glucosidase Inhibitor. Pharmacognosy Journal, 11 (2). pp. 358-361. ISSN 0975-3575

	Text 2.JURNAL_ELISA Test on Cordia myxa L. Leaf Extract for α-Glucosidase.pdf Download (454kB)
	Text Similarity_Check_ELISA .pdf Download (1MB)

Abstract

Material: ELISA Kit, Ethanol 96%, Colomn Chromatography, n-hexane, ethyl acetate, Glocobay®. Method: Sample from Cordia myxa L. leaf extracted by ethanol 96% then evaporated to get the sticky extract. The sticky extract of Cordia myxa L. leaf fractionated by column chromatography with n-hexane, n-hexane: ethyl acetate (90:10; 80:20; 75:25; 70:30; 65:35; 60:40; 55:45; 50:50) Assay: The fractions assayed by ELISA (Enzyme-Linked Immunosorbent Assay) with acarbose (Glucobay ®) as the comparator. Result: The results showed that the n-hexane fraction is the highest potency on inhibited α-glucosidase with the noncompetitive mechanism. The IC50 of n-hexane fraction is 0.53 ppm been while the acarbose is 6.85 ppm. Conclusion: The n-hexane fraction of Cordia myxa L. leaf has the highest potency to use for possible decrease blood glucose level.

Item Type:	Article
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	FAKULTAS FARMASI
Depositing User:	Unnamed user with email admin@umi.ac.id
Date Deposited:	29 May 2023 02:07
Last Modified:	29 May 2023 02:20
URI:	http://repository.umi.ac.id/id/eprint/2554

Actions (login required)

View Item