Evaluation of Antioxidant Activity of Vernonia amygdalina Leaves and Its Flavonoid-Phenolic Content

Fawwaz, Muammar (2023) Evaluation of Antioxidant Activity of Vernonia amygdalina Leaves and Its Flavonoid-Phenolic Content. Indonesian Journal of Pharmaceutical Science and Technology, 10 (2). pp. 104-110. ISSN 2406-856X

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Abstract

Bitter leaf, Vernonia amygdalina (VA), is used in various alternative medicine in several countries, including Indonesia. This study aims to evaluate the antioxidant activity of VA leaves by three different in vitro methods. The antioxidant activity was evaluated by radical scavenging of 1,1-diphenyl-2- picryl-hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC). The antioxidant capacity by FRAP and CUPRAC methods were expressed as mg of quercetin equivalents (QE) per g extract (mgQE/g) and mg of ascorbic acid equivalents (AAE) per g extract (mgAAE/g), respectively. All methods were measured by UV-Visible spectrophotometry at maximum wavelength. The results exhibited that VA leaves ethanolic extract contains flavonoidphenolic. The VA leaves ethanolic extract has an antioxidant activity by scavenging the DPPH method with the half inhibition concentration (IC50) was 8,22 μg/mL. The antioxidant capacity of VA leaves ethanolic extract by FRAP and CUPRAC methods was 21,03 mgQE/g and 160,64 mgAAE/g extract, respectively. The VA leaves ethanolic extract has a powerful antioxidant potency which might correlate to the flavonoid-phenolic content in the extract.

Item Type:	Article
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	FAKULTAS FARMASI
Depositing User:	Unnamed user with email admin@umi.ac.id
Date Deposited:	04 Jul 2023 00:49
Last Modified:	04 Jul 2023 00:49
URI:	http://repository.umi.ac.id/id/eprint/2940

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