DNA barcoding of Telmatherinidae family in Lake Towuti, South Sulawesi, Indonesia

Hadijah, St (2019) DNA barcoding of Telmatherinidae family in Lake Towuti, South Sulawesi, Indonesia. AACL Bioflux, 12 (4). pp. 1208-1215. ISSN 1844-9166, 1844-8143

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Abstract

Telamtherinidae is a family of endemic fish in South Sulawesi. The study is aimed at identifying the molecular gene of the endemic fish of the Telmatherinidae family in Towuti Lake, South Sulawesi, to identify and analyze genetic diversity, genetic marking, genetic distances, genetic characterization, and dendrogram of the fish. DNA barcoding in this research used the Cytochrome c Oxidase I (COI) gene. Amplification of mitochondrial COI gene regions was conducted by using COI Fish F2 and COI Fish R 2 primer. Data analysis, total isolation of DNA, Polymerase Chain Reaction (PCR), Electrophoresis, gel purification, and sequencing Basic Local Alignment Search Tool (BLAST) were performed. The results showed that DNA sequence was 681 bp. Meanwhile, analysis of dendrogram suggested that the fish of Telmatherinidae family in Towuti Lake are similar to the fish in the Paratherina, Telmatherina and Tominanga genera. The genus Telmatherina including Telmatherina celebensis, Telmatherina bonti, and Telmatherina opudi showed 85% significant shared similarity with Atherina sp. Only 84% similarity of genus Telmatherina was found in Pristigenys alta, Parexocoetus brachypterus, Cypselurus hiraii, and Hypoatherina tsurugae. Meanwhile, genus Paratherina: Paratherina striata and Paratherina wolterecki have 83-84% similarity with Scorpis lineolata, Hyporhamphus affinis, Cypselurus hiraii, Parexocoetus brachypterus, and Pristigenys alta. In addition, genus Tominanga: Tominanga sanguicauda has 84% similarity with Hypoatherina tsurugae, Cypselurus hiraii, Prognichthys sealei, Hyporhamphus affinis, and Pristigenys alta. Key Words: genetic diversity, Telmatherinidae, COI gene, dendrogram, Lake Towuti.

Item Type: Article
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: FAKULTAS PERIKANAN > ILMU KELAUTAN
Depositing User: Unnamed user with email admin@umi.ac.id
Date Deposited: 10 Jul 2023 00:42
Last Modified: 10 Jul 2023 00:42
URI: http://repository.umi.ac.id/id/eprint/3089

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