Comparative Study of HPTLC Fingerprint of β-Asarone Content between Leaves and Rhizome of Acorus calamus L.

Abstract: ß-asarone as a major compound of Sweet Flag (Acorus calamus L.) family acoraceae. ß-asarone accumulate on rhizome and distribute generally on the other part of plant including on leaves. The aim of this study is determine of ß-asarone contents of Sweet Flag (A. Calamus L.) between rhizome and leaves by HPTLC method. Rhizome and leaves of Sweet Flag (A. Calamus L.) extracted by Stahl steam distillation. Extract rendemen is 2.4% mL/g from rhizome and 4.5% mL/g from leaves. Extract and ß-asarone eluted by nhexane:aethyl acetat (9:1). TLC plate scaned at maximum wavelength 298 nm. The result of ß-asarone contens between rhizome and leave are 5.1386 µg/mL equivalent 0.23246 µg/g and 4.21866 µg/mL equivalent 0.22499 µg/g respectively. Key word: ß-asarone, Acorus calamus L., HPTLC.