Comparative Study of Antioxidant Activity of Red and Green Varieties of Grape Seed (Vitis Vinifera L.) using DPPH Reduction Method

Abstract: ß-asarone as a major compound of Sweet Flag (Acorus calamus L.) family acoraceae. ß-asarone accumulate on rhizome and distribute generally on the other part of plant including on leaves. The aim of this study is determine of ß-asarone contents of Sweet Flag (A. Calamus L.) between rhizome and leaves by HPTLC method. Rhizome and leaves of Sweet Flag (A. Calamus L.) extracted by Stahl steam distillation. Extract rendemen is 2.4% mL/g from rhizome and 4.5% mL/g from leaves. Extract and ß-asarone eluted by n- hexane:aethyl acetat (9:1). TLC plate scaned at maximum wavelength 298 nm. The result of ß-asarone contens between rhizome and leave are 5.1386 μg/mL equivalent 0.23246 μg/g and 4.21866 μg/mL equivalent 0.22499 μg/g respectively. Key word: ß-asarone, Acorus calamus L., HPTLC.