Genetic Diversity Population of Beloso (Glossogobius aureus) in Lake Tempe, Sulawesi, Indonesia
Abstrak
Beloso fish (Glossogobius aureus) is a native species in Lake Tempe. There has been a decline in
production and Beloso size, so that preservation should be done. The objective of this research was to
determine the genetic diversity of various populations of Beloso fish. The research was conducted in Lake
Tampe. Organ samples are meat. Beloso genetic diversity was measured with a set of extraction and
purification of genomic tools, mt-DNA amplification and electrophoresis. Results of this study found that mt-
DNA PCR product read at length 600 bp gene. The length of DNA fragments of beloso fish were sequenced
with primer F and primer R is the range between 456 - 607 bp and 565 - 604 bp, respectively. Genetic distance
of individual Beloso (Glossogobius aureus) in Lake Tempe is around 0.0012 - 0.0170. The genetic diversity of
Beloso was very low leading to the lower income of the communities around Lake Tempe with a lower
population and diversity of Beloso fish.
Key words: Glossogobius aureus Purification Genome Mitochondrial DNA Amplification
Electrophoresis
production and Beloso size, so that preservation should be done. The objective of this research was to
determine the genetic diversity of various populations of Beloso fish. The research was conducted in Lake
Tampe. Organ samples are meat. Beloso genetic diversity was measured with a set of extraction and
purification of genomic tools, mt-DNA amplification and electrophoresis. Results of this study found that mt-
DNA PCR product read at length 600 bp gene. The length of DNA fragments of beloso fish were sequenced
with primer F and primer R is the range between 456 - 607 bp and 565 - 604 bp, respectively. Genetic distance
of individual Beloso (Glossogobius aureus) in Lake Tempe is around 0.0012 - 0.0170. The genetic diversity of
Beloso was very low leading to the lower income of the communities around Lake Tempe with a lower
population and diversity of Beloso fish.
Key words: Glossogobius aureus Purification Genome Mitochondrial DNA Amplification
Electrophoresis