Analysis of Total Phenolic and Flavonoid Content of Patikala Leaf Etract (Etlingera elatior (Jack). Smith

ABSTRACT Patikala (Etlingera elatior (Jack) Smith) is an herbal plant that belongs to the family of Zingiberaceae with the genus Alpinieae. This plant contains bioactive compounds, such as; flavonoids, terpenoids, saponins, tannins, carbohydrates, and phenolics. So we tested the total phenolic and flavonoid content of patikala leaf extract using various types of solvent. Total flavonoid testing used 1,2% AlCl3 and 120 mM potassium acetate reagent with quarsetin comparator, while total phenolic using Folin-Ciocalteau (1: 9) and NaHCO3 7.5% reagent with gallic acid comparator. The determination of the content was done using the UV-Vis spectrophotometric method. The results showed that the total flavonoid content of water extract, ethanol extract and purification leaf extract were 24.31 mgQE/g, 26.41 mgQE/g, and 46.5 mgQE/gr, respectively. While the total phenolic content of water extract, ethanol extract and purine leaf purification extract were 90.38 mgGAE/g, 98.94 mgGAE/g, and 228.54 mgGAE/g, respectively. Key words : Patikala (Etlingera elatior (Jack) Smith), flavonoid, phenolic, UV-Vis spectrophotometric