Antibacterial activity of endophytic fungi isolated from Portulaca Oleracea L

Portulaca oleracea L is an annual grassy plant that is distributed in many parts of the world. Endophytes represent a complex community of microorganisms colonizing asymptomatically internal tissues of higher plants. Several reports have shown that endophytes enhance the fitness of their host plants by direct production of bioactive secondary metabolites. This study investigated the antibacterial activity of endophytes isolated from Portulaca Oleracea L. Isolation was carried out on potato dextrose agar chloramphenicol. Antibacterial activity using disc diffusion methods. Isolation results obtained as many as 8 endophytic fungi isolate with code IFK 1-IFK 8 isolates. The antibacterial activity test showed that the isolates with the IFK 6 had the best activity in inhibiting the growth of Staphylococcus aureus (14,03 mm) and Escherichia coli (15,93 mm).Then isolate IFK6 identified by PCR test. The results showed that the molecular characteristics of Portulaca oleracea isolates of IFK6 were genus Aspergillus Sp had similar species based on the results of sequencing, namely Aspergillus versicolor. Based on macroscopic and molecular characteristics, IFK6 isolates had similarities with the fungus Aspergillus versicolor and has antibacterial activity against Staphylococcus aureus and Escherichia coli.