AKTIVITAS INHIBISI ENZIM ALFA-GLUKOSIDASE DARI EKSTRAK BUNGA KERSEN (Muntingia calabura L.) SECARA IN VITRO

RESQINAH AZZAHRA. Aktivitas Inhibisi Enzim Alfa-Glukosidase Dari
Ekstrak Bunga Kersen (Muntingia calabura L.) Secara In Vitro. (Dibimbing
oleh St. Maryam dan Masdiana Tahir).
Tumbuhan kersen merupakan salah satu tumbuhan yang sangat
potensial untuk dimanfaatkan karena memiliki beberapa kandungan bioaktif
yang bermanfaat untuk kesehatan salah satunya ialah flavonoid. Penelitian
ini bertujuan untuk mengetahui aktivitas inhibisi enzim alfa-glukosidase dari
ekstrak etanol bunga kersen (Muntingia calabura L.) secara in vitro
menggunakan alat instrument Microplate reader. Metode penelitian ini
diawali dengan penyiapan sampel dengan cara dimaserasi menggunakan
pelarut etanol 96% lalu dilakukan uji penghambatan aktivitas enzim alfaglukosidase
menggunakan substrat p-nitrofenil-α-D-glukopiranosida
(PNPG) dan akarbosa sebagai pembanding. Potensi suatu senyawa dalam
menghambat aktivitas enzim dapat diketahui melalui perhitungan IC50.
Hasil penelitian menunjukkan bahwa ekstrak etanol bunga kersen
(Muntingia calabura L.) memiliki nilai IC50 sebesar 158,262 μg/mL dan nilai
IC50 akarbosa sebesar 0,256 μg/mL.
Kata Kunci: Bunga kersen (Muntingia calabura L.), alfa-glukosidase, IC50

RESQINAH AZZAHRA. In Vitro Inhibition Assay of Alpha-Glucosidase
of Jamaica Cherry Extract (Muntingia calabura L.). (Supervised by St.
Maryam and Masdiana Tahir).
The Muntingia calabura L., commonly referred to as Jamaica cherry,
is recognized for its significant potential in therapeutic applications, primarily
attributed to its rich bioactive constituents, notably flavonoids. The primary
objective of this study was to elucidate the inhibitory activities of alphaglucosidase
enzyme derived from the ethanol extract of Jamaica cherry
using the microplate reader instrument. The experimental methodology
entailed an initial phase of sample preparation, wherein Jamaica cherry
underwent maceration with a 96% ethanol. Subsequent phases involved
assessing the inhibitory potential of alpha-glucosidase, employing pnitrophenyl-
α-D-glucopyranoside (PNPG) as the substrate, with acarbose
serving as the benchmark for comparison. The inhibitory capacity of a
compound was quantitatively represented through its IC50 value. The
findings of this research revealed that the ethanol extract of Jamaica cherry
exhibited an IC50 value of 158.262 μg/mL, in contrast to acarbose, which
demonstrated an IC50 of 0.256 μg/mL.
Keywords: Jamaica cherry (Muntingia calabura L.), alpha-glucosidase,
IC50.