Pengaruh Metode Ekstraksi Terhadap Senyawa Flavonoid Total Ekstrak Etanol Daun Patikan Kebo (Ephorbia hirta)
Abstrak
Euphorbia (Euphorbia hirta) merupakan tanaman yang banyak digunakan dalam pengobatan tradisional. Salah satu senyawa aktif yang terdapat pada ekstrak etanol daun Euphorbia adalah flavonoid. Penelitian ini bertujuan untuk mengetahui pengaruh variasi metode ekstraksi terhadap kandungan flavonoid total pada ekstrak daun Euphorbia. Ekstraksi sampel menggunakan metode perkolasi dan refluks dengan nilai rendemen untuk metode perkolasi sebesar 4,3% dan metode refluks sebesar 5,3%. Analisis kualitatif sampel menggunakan Kromatografi Lapis Tipis (KLT). Penentuan kadar flavonoid total ekstrak etanol daun Euphorbia diperoleh dari data spektrofotometer UV-Vis. Panjang gelombang maksimum yang digunakan adalah 429 nm. Hasil penelitian menunjukkan bahwa kandungan total flavonoid ekstrak etanol daun Euphorbia untuk metode perkolasi adalah 2,32% dan untuk metode refluks adalah 3,21%.
Kata Kunci:
daun Euphorbia (Euphorbia hirta); variasi metode ekstraksi; flavonoid total;spektrofotometer UV-Vis
Euphorbia (Euphorbia hirta) is a plant widely used in traditional medicine. One of the active compounds found in the ethanol extract of Euphorbia leaves is flavonoids. This research aimed to determine the effect of extraction method variation on total flavonoid content in Euphorbia leaf extract. The sample extraction was obtained by percolation and reflux with yield value for the percolation method of 4,3% and reflux method 0f 5,3%. The qualitative analysis of the samples using Thin Layer Chromatography (TLC). Determination of the total flavonoid content of ethanol extract of Euphorbia leaf is obtained from UV-Vis spectrophotometer data. It measured at the maximum wavelength of 429 nm. The result shows that the total flavonoid content of ethanol extract of Euphorbia leaf for the percolation method is 2.32% extract and for the reflux method is 3.21% extract.
Keywords:
Euphorbia leaves (Euphorbia hirta), extraction method variation, total flavonoid, UV-Vis spectrophotometer
Kata Kunci:
daun Euphorbia (Euphorbia hirta); variasi metode ekstraksi; flavonoid total;spektrofotometer UV-Vis
Euphorbia (Euphorbia hirta) is a plant widely used in traditional medicine. One of the active compounds found in the ethanol extract of Euphorbia leaves is flavonoids. This research aimed to determine the effect of extraction method variation on total flavonoid content in Euphorbia leaf extract. The sample extraction was obtained by percolation and reflux with yield value for the percolation method of 4,3% and reflux method 0f 5,3%. The qualitative analysis of the samples using Thin Layer Chromatography (TLC). Determination of the total flavonoid content of ethanol extract of Euphorbia leaf is obtained from UV-Vis spectrophotometer data. It measured at the maximum wavelength of 429 nm. The result shows that the total flavonoid content of ethanol extract of Euphorbia leaf for the percolation method is 2.32% extract and for the reflux method is 3.21% extract.
Keywords:
Euphorbia leaves (Euphorbia hirta), extraction method variation, total flavonoid, UV-Vis spectrophotometer
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